Hotspot

trnT-GGU-psbD

LSC · 31356-32683 · 1328 bp

Back to Caroxylon

Pi 0.0232

MarkerSeek score 67.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1328
variable_sites: 46
pi: 0.0232
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1482-1509 bp	1.0	80.8
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1485-1512 bp	1.0	80.6
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	55.0	50.0	58.1	57.6	1481-1508 bp	1.0	79.6
trnT-GGU-psbD_p4	TCCGATAAGGGGCTTTTTGG	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1438-1444 bp	0.666667	64.3
trnT-GGU-psbD_p5	CGATAAGGGGCTTTTTGGGT	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.2	58.2	1436-1442 bp	0.666667	64.1