Hotspot

psbM-trnD-GUC

LSC · 29054-30332 · 1279 bp

Back to Caroxylon

Pi 0.0214

MarkerSeek score 67.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1279
variable_sites: 40
pi: 0.0214
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCATTTTGGCTGACCGTTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	56.4	58.6	1430-1443 bp	1.0	67.1
psbM-trnD-GUC_p2	AATCATTTTGGCTGACCGTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	56.2	58.6	1432-1445 bp	1.0	65.3
psbM-trnD-GUC_p3	ATCATTTTGGCTGACCGTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	56.2	58.6	1431-1444 bp	1.0	65.3
psbM-trnD-GUC_p4	TCATTTTGGCTGACCGTTTTT	TCAATTGGTTAGAGCACCGC	38.1	50.0	57.2	58.6	1430-1443 bp	1.0	65.1
psbM-trnD-GUC_p5	TTTGGCTGACCGTTTTTACA	TCAATTGGTTAGAGCACCGC	40.0	50.0	56.1	58.6	1426-1439 bp	1.0	64.4