Hotspot

trnC-GCA-petN

LSC · 27677-28156 · 480 bp

Back to Caroxylon

Pi 0.0299

MarkerSeek score 67.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 480
variable_sites: 21
pi: 0.0299
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	GCCCAAGCGAGACTTACAAT	45.0	50.0	57.9	58.3	590-594 bp	1.0	80.5
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACAAT	50.0	50.0	58.1	58.3	534-538 bp	1.0	80.5
trnC-GCA-petN_p3	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	652-656 bp	1.0	79.5
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	596-600 bp	1.0	79.5
trnC-GCA-petN_p5	TTTTGTATCGTTTTGGCGGC	ATTAAAGCAGCCCAAGCGAG	45.0	50.0	57.9	58.9	599-603 bp	1.0	75.3