Hotspot

trnS-GCU-trnG-GCC

LSC · 7945-8829 · 885 bp

Back to Caroxylon

Pi 0.0324

MarkerSeek score 65.00

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 43
pi: 0.0324
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	GAATGGCTCGTACAACGGAT	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.1	58.0	1687-1705 bp	1.0	82.0
trnS-GCU-trnG-GCC_p2	GCTCGTACAACGGATTAGCA	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.1	58.0	1682-1700 bp	1.0	81.8
trnS-GCU-trnG-GCC_p3	GAATGGCTCGTACAACGGAT	CGTTAGCTTGGAAGGCTAGG	50.0	55.0	58.1	58.1	1684-1702 bp	1.0	81.8
trnS-GCU-trnG-GCC_p4	GAATGGCTCGTACAACGGAT	GTAATCAAACCCGGAGACCC	50.0	55.0	58.1	58.0	1021 bp	0.666667	66.0
trnS-GCU-trnG-GCC_p5	GCTCGTACAACGGATTAGCA	GTAATCAAACCCGGAGACCC	50.0	55.0	58.1	58.0	1016 bp	0.666667	65.9