Hotspot

ycf1

SSC · 108890-114079 · 5190 bp

Back to Carlina

Pi 0.0111

MarkerSeek score 60.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5190
variable_sites: 104
pi: 0.0111
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CACCAGCTTCGAACAAGAA	GCGCATAGCTCCTTTGACTA	47.4	50.0	55.8	57.8	180 bp	1.0	56.4
ycf1_p2	CACCAGCTTCGAACAAGAA	ATGGCTCGACGAAAATCTGG	47.4	50.0	55.8	58.3	161 bp	1.0	56.0
ycf1_p3	CACCAGCTTCGAACAAGAA	GCTCCTTTGACTATGGCTCG	47.4	55.0	55.8	58.4	173 bp	1.0	55.0
ycf1_p4	CACCAGCTTCGAACAAGAA	CCTTTGACTATGGCTCGACG	47.4	55.0	55.8	58.4	170 bp	1.0	55.0
ycf1_p5	CACCAGCTTCGAACAAGAA	CTTTGACTATGGCTCGACGA	47.4	50.0	55.8	57.4	169 bp	1.0	54.0