Hotspot

clpP

LSC · 70054-72072 · 2019 bp

Back to Carlina

Pi 0.00644

MarkerSeek score 62.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2019
variable_sites: 24
pi: 0.00644
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCACGAATTGACCCGGAATC	GCCTTTGTTACGTTTCCACA	50.0	45.0	58.0	56.9	2111-2122 bp	1.0	71.7
clpP_p2	CTCGGCAGGGGTAATTTCAA	GCCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2132-2143 bp	1.0	70.8
clpP_p3	AACTCGGCAGGGGTAATTTC	GCCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2134-2145 bp	1.0	70.8
clpP_p4	TCACGAATTGACCCGGAATC	AGCCTTTGTTACGTTTCCACA	50.0	42.9	58.0	58.4	2112-2123 bp	1.0	70.0
clpP_p5	TCACGAATTGACCCGGAATC	AGCCTTTGTTACGTTTCCAC	50.0	45.0	58.0	56.6	2112-2123 bp	1.0	69.3