Hotspot

trnL-UAG-rpl32

SSC · 123672-124530 · 859 bp

Back to Carlina

Pi 0.00931
MarkerSeek score 60.60
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
859
variable_sites
15
pi
0.00931
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC AAGGAAAGGATATTGGGCGG 50.0 50.0 58.0 57.6 989-992 bp 1.0 78.5
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA AAGGAAAGGATATTGGGCGG 50.0 50.0 58.0 57.6 990-993 bp 1.0 78.5
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 972-975 bp 1.0 78.3
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 973-976 bp 1.0 78.3
trnL-UAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA AAGGAAAGGATATTGGGCGG 50.0 50.0 57.9 57.6 999-1002 bp 1.0 77.5