Hotspot

rpl16-rps3

LSC · 82742-82895 · 154 bp

Back to Carlina

Pi 0.0141

MarkerSeek score 67.30

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 154
variable_sites: 4
pi: 0.0141
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAACCAACGAGTCACACACT	GAGGGTAGGGTTCCACTACA	45.0	55.0	57.6	57.8	280-302 bp	1.0	80.0
rpl16-rps3_p2	AAACCAACGAGTCACACACT	AGAGGGTAGGGTTCCACTAC	45.0	55.0	57.6	57.5	281-303 bp	1.0	77.5
rpl16-rps3_p3	AAACCAACGAGTCACACACT	AGGGTAGGGTTCCACTACAA	45.0	50.0	57.6	57.3	279-301 bp	1.0	76.1
rpl16-rps3_p4	AACGAGTCACACACTAAGCA	GAGGGTAGGGTTCCACTACA	45.0	55.0	57.1	57.8	275-297 bp	1.0	76.1
rpl16-rps3_p5	AAACCAACGAGTCACACACT	GAGAGGGTAGGGTTCCACTA	45.0	55.0	57.6	57.2	282-304 bp	1.0	75.2