Hotspot

rpl14-rpl16

LSC · 81158-81290 · 133 bp

Back to Carlina

Pi 0.026

MarkerSeek score 74.50

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 6
pi: 0.026
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACACATCAATTCTCGAGCCC	AATAGCAGCGTCCAAAATGC	50.0	45.0	58.0	57.4	240-252 bp	1.0	80.1
rpl14-rpl16_p2	TCGACGATTGCTAGCTCCTA	AATAGCAGCGTCCAAAATGC	50.0	45.0	58.0	57.4	270-282 bp	1.0	79.5
rpl14-rpl16_p3	ACACATCAATTCTCGAGCCC	GCGTCCAAAATGCCTATACG	50.0	50.0	58.0	57.3	233-245 bp	1.0	79.5
rpl14-rpl16_p4	TCGACGATTGCTAGCTCCTA	GCGTCCAAAATGCCTATACG	50.0	50.0	58.0	57.3	263-275 bp	1.0	78.8
rpl14-rpl16_p5	CCCGCTGTTATCTGCTACAT	AATAGCAGCGTCCAAAATGC	50.0	45.0	57.5	57.4	222-234 bp	1.0	76.4