Hotspot

trnT-UGU-trnL-UAA

LSC · 46687-47223 · 537 bp

Back to Carlina

Pi 0.0177
MarkerSeek score 66.30
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
537
variable_sites
17
pi
0.0177
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 582-610 bp 1.0 83.1
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 614-642 bp 1.0 81.9
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 576-604 bp 1.0 81.9
trnT-UGU-trnL-UAA_p4 GGAATCGAACCGATGACCAT CGTCTACCGATTTCGCCATA 50.0 50.0 57.8 57.6 608-636 bp 1.0 80.7
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG CGTAGCGTCTACCGATTTCG 55.0 55.0 57.9 58.6 581-609 bp 1.0 80.2