Hotspot

atpF-atpA

LSC · 28392-28456 · 65 bp

Back to Carlina

Pi 0.0282
MarkerSeek score 61.00
Primer available yes
Label atpF-atpA

Feature Metadata

label_name
atpF-atpA
feature_type
igs
region
LSC
length_bp
65
variable_sites
3
pi
0.0282
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpA_p1 TTACATTGAGCGTGAGAGCC CACCCGCCATTACTTCATCA 50.0 50.0 58.0 58.0 729-732 bp 1.0 80.6
atpF-atpA_p2 TTACATTGAGCGTGAGAGCC CCGTTTCGGCTACCACAATA 50.0 50.0 58.0 58.0 1260-1263 bp 1.0 80.4
atpF-atpA_p3 ATCAGTTTCGCGTGAATGGA CCGTTTCGGCTACCACAATA 45.0 50.0 57.9 58.0 1025-1028 bp 1.0 79.9
atpF-atpA_p4 TCAGTTTCGCGTGAATGGAT CCGTTTCGGCTACCACAATA 45.0 50.0 57.9 58.0 1024-1027 bp 1.0 79.9
atpF-atpA_p5 TCATGTTTGGTTCGGGAAGG CCGTTTCGGCTACCACAATA 50.0 50.0 58.1 58.0 1224-1227 bp 1.0 79.8