Hotspot

petN-psbM

LSC · 10399-10900 · 502 bp

Back to Carlina

Pi 0.00863

MarkerSeek score 64.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 502
variable_sites: 8
pi: 0.00863
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTT	50.0	40.0	58.2	55.6	661-668 bp	1.0	61.2
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTTT	50.0	38.1	58.2	56.4	661-668 bp	1.0	59.5
petN-psbM_p3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTT	50.0	40.0	57.1	55.6	662-669 bp	1.0	55.3
petN-psbM_p4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTT	50.0	40.0	58.9	55.6	653-660 bp	1.0	55.2
petN-psbM_p5	GCTTGGGCTGCTTTAATGGT	TGCTACTGCACTGTTCATTT	50.0	40.0	59.1	55.6	650-657 bp	1.0	53.6