Hotspot

trnS-GCU-trnC-GCA

LSC · 8608-9426 · 819 bp

Back to Carlina

Pi 0.013
MarkerSeek score 65.80
Primer available yes
Label trnS-GCU-trnC-GCA

Feature Metadata

label_name
trnS-GCU-trnC-GCA
feature_type
igs
region
LSC
length_bp
819
variable_sites
20
pi
0.013
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnC-GCA_p1 ACGGAAAGAGAGGGATTCGA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.2 58.1 970-980 bp 1.0 79.9
trnS-GCU-trnC-GCA_p2 ACGGAAAGAGAGGGATTCGA AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 58.4 952-962 bp 1.0 77.8
trnS-GCU-trnC-GCA_p3 CTTTCGTCCACTCAGCCATC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.6 58.1 906-916 bp 1.0 76.2
trnS-GCU-trnC-GCA_p4 AACGGAAAGAGAGGGATTCG CCGGATTTGAACTGGGGAAA 50.0 50.0 57.3 58.1 971-981 bp 1.0 75.8
trnS-GCU-trnC-GCA_p5 CTTTCGTCCACTCAGCCATC AAAAGGATTTGCAGTCCCCC 55.0 50.0 58.6 58.4 888-898 bp 1.0 74.0