Hotspot

trnK-UUU-rps16

LSC · 4375-5180 · 806 bp

Back to Carlina

Pi 0.00393
MarkerSeek score 60.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
806
variable_sites
6
pi
0.00393
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 941-942 bp 1.0 76.7
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 940-941 bp 1.0 76.7
trnK-UUU-rps16_p3 TCAGTCGTGGTCTTCCAAAC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1026-1029 bp 1.0 76.2
trnK-UUU-rps16_p4 CAGTCGTGGTCTTCCAAACT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1025-1028 bp 1.0 76.1
trnK-UUU-rps16_p5 TCCTAGCCGCACTTAAAAGC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.3 58.3 956-957 bp 1.0 75.9