Hotspot

rpl32-trnL-UAG

SSC · 114533-115516 · 984 bp

Back to Carissa

Pi 0.00102

MarkerSeek score 59.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 984
variable_sites: 1
pi: 0.00102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGAAGGGATATTGGACAGCA	TTAGAAGATGACATGCCGCC	50.0	50.0	57.0	58.0	1172-1178 bp	1.0	71.9
rpl32-trnL-UAG_p2	GGAAGGGATATTGGACAGCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.0	57.9	1118-1124 bp	1.0	71.3
rpl32-trnL-UAG_p3	GGAAGGGATATTGGACAGCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.0	57.8	1111-1117 bp	1.0	70.8
rpl32-trnL-UAG_p4	GGAAGGGATATTGGACAGCA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.0	57.8	1117-1123 bp	1.0	70.7
rpl32-trnL-UAG_p5	GGAAGGGATATTGGACAGCA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.0	57.8	1116-1122 bp	1.0	70.7