Hotspot

rpl22-rps19

LSC · 85315-85378 · 64 bp

Back to Carissa

Pi 0.0156

MarkerSeek score 61.50

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 1
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	ATAGGAACGTCCGCGAATTT	GGAAAGGAGCATGTGCCTAT	45.0	50.0	57.7	57.6	301 bp	1.0	75.0
rpl22-rps19_p2	CATAGGAACGTCCGCGAATT	GGAAAGGAGCATGTGCCTAT	50.0	50.0	58.4	57.6	302 bp	1.0	74.0
rpl22-rps19_p3	ATAGGAACGTCCGCGAATTT	GCGATAATAGATCTCGGCGT	45.0	50.0	57.7	57.5	204 bp	1.0	73.6
rpl22-rps19_p4	CATAGGAACGTCCGCGAATT	GCGATAATAGATCTCGGCGT	50.0	50.0	58.4	57.5	205 bp	1.0	72.6
rpl22-rps19_p5	GTCCGCGAATTTGGTCAATT	GGAAAGGAGCATGTGCCTAT	45.0	50.0	57.4	57.6	293 bp	1.0	72.4