Hotspot

rpl20-rps12

LSC · 70380-71173 · 794 bp

Back to Carissa

Pi 0.00252

MarkerSeek score 60.20

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 794
variable_sites: 2
pi: 0.00252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGGGCTCCTCGAAA	CTCAACGTCGAGGAACATGT	50.0	50.0	57.9	57.9	915 bp	1.0	78.7
rpl20-rps12_p2	TTGAATGGGCTCCTCGAAAG	CTCAACGTCGAGGAACATGT	50.0	50.0	57.9	57.9	914 bp	1.0	78.7
rpl20-rps12_p3	TCCTCGAAAGCTTGATGCAA	CTCAACGTCGAGGAACATGT	45.0	50.0	57.8	57.9	904 bp	1.0	78.2
rpl20-rps12_p4	CTCCTCGAAAGCTTGATGCA	CTCAACGTCGAGGAACATGT	50.0	50.0	58.3	57.9	905 bp	1.0	77.5
rpl20-rps12_p5	TTGAATGGGCTCCTCGAAAG	GAGGAACATGTACTCGGGTG	50.0	55.0	57.9	57.7	905 bp	1.0	76.9