Hotspot

psbM-trnD-GUC

LSC · 30538-31425 · 888 bp

Back to Carissa

Pi 0.00227

MarkerSeek score 60.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 888
variable_sites: 2
pi: 0.00227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	1007-1013 bp	1.0	68.6
psbM-trnD-GUC_p2	AGAAAGGCGGTAGGAACTAGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.9	58.5	1011-1017 bp	1.0	68.0
psbM-trnD-GUC_p3	AGTAGAAAGGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.2	58.5	1014-1020 bp	1.0	67.4
psbM-trnD-GUC_p4	AGTAGAAAGGCGGTAGGAAC	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.7	58.5	1014-1020 bp	1.0	66.1
psbM-trnD-GUC_p5	GTAGAAAGGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.7	58.5	1013-1019 bp	1.0	66.1