Hotspot

atpH-atpI

LSC · 13535-14722 · 1188 bp

Back to Carissa

Pi 0.000842

MarkerSeek score 47.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1188
variable_sites: 1
pi: 0.000842
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1275-1276 bp	1.0	75.7
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	1276-1277 bp	1.0	74.5
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.0	57.3	1260-1261 bp	1.0	73.9
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGC	45.0	55.0	58.0	58.9	1248-1249 bp	1.0	72.9
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.2	57.3	1261-1262 bp	1.0	72.8