Hotspot

trnK-UUU-rps16

LSC · 4356-5131 · 776 bp

Back to Carissa

Pi 0
MarkerSeek score 60.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
776
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC GAAAAGGGGGCTCAACCTAC 55.0 55.0 57.9 58.2 954-967 bp 1.0 79.1
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GAAAAGGGGGCTCAACCTAC 50.0 55.0 58.2 58.2 893-906 bp 1.0 78.5
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GAAAAGGGGGCTCAACCTAC 50.0 55.0 58.2 58.2 892-905 bp 1.0 78.5
trnK-UUU-rps16_p4 TCCTAGCCGCACTTAAAAGC GAAAAGGGGGCTCAACCTAC 50.0 55.0 58.3 58.2 908-921 bp 1.0 77.7
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA GAAAAGGGGGCTCAACCTAC 50.0 55.0 58.3 58.2 902-915 bp 1.0 77.6