Hotspot

ycf1

SSC · 122465-127732 · 5268 bp

Back to Carduus

Pi 0.00313

MarkerSeek score 54.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5268
variable_sites: 32
pi: 0.00313
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAGATCCAAACGCTCAAACA	ACAACCGATCCGAAAACTAAGA	40.0	40.9	55.9	57.7	214 bp	1.0	46.8
ycf1_p2	AGATCCAAACGCTCAAACAT	ACAACCGATCCGAAAACTAAGA	40.0	40.9	55.6	57.7	213 bp	1.0	44.9
ycf1_p3	AAGATCCAAACGCTCAAACA	CCGATCCGAAAACTAAGAATCG	40.0	45.5	55.9	57.3	210 bp	1.0	43.4
ycf1_p4	AAGATCCAAACGCTCAAACAT	ACAACCGATCCGAAAACTAAGA	38.1	40.9	56.4	57.7	214 bp	1.0	43.2
ycf1_p5	AGATCCAAACGCTCAAACATT	ACAACCGATCCGAAAACTAAGA	38.1	40.9	56.4	57.7	213 bp	1.0	43.2