Hotspot

petD

LSC · 75691-76864 · 1174 bp

Back to Carduus

Pi 0.00128

MarkerSeek score 56.60

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1174
variable_sites: 3
pi: 0.00128
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ATTTCCCTTGAACTCTGCAA	TGAAGCGACTGTTCCCTAGA	40.0	50.0	55.1	58.1	1308 bp	1.0	56.6
petD_p2	TTTCCCTTGAACTCTGCAAT	TGAAGCGACTGTTCCCTAGA	40.0	50.0	55.1	58.1	1307 bp	1.0	56.6
petD_p3	ATTTCCCTTGAACTCTGCAA	CTTTGAAGCGACTGTTCCCT	40.0	50.0	55.1	58.1	1311 bp	1.0	56.4
petD_p4	TTTCCCTTGAACTCTGCAAT	CTTTGAAGCGACTGTTCCCT	40.0	50.0	55.1	58.1	1310 bp	1.0	56.4
petD_p5	GGATATTTCCCTTGAACTCTGC	TGAAGCGACTGTTCCCTAGA	45.5	50.0	56.8	58.1	1312 bp	1.0	54.2