Hotspot

ndhF

SSC · 108471-110705 · 2235 bp

Back to Carduus

Pi 0.00157

MarkerSeek score 58.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 7
pi: 0.00157
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGTTTCGATGCAACAGCAAG	GAGGTAAGATCCGGTGAATCG	45.0	52.4	57.6	58.0	2380 bp	1.0	68.9
ndhF_p2	TGTTTCGATGCAACAGCAAG	AGGTAAGATCCGGTGAATCG	45.0	50.0	57.6	56.8	2379 bp	1.0	67.6
ndhF_p3	TGTTTCGATGCAACAGCAAG	CGAAAGAGGTAAGATCCGGTG	45.0	52.4	57.6	58.2	2385 bp	1.0	67.5
ndhF_p4	TGTTTCGATGCAACAGCAAG	CGAAAGAGGTAAGATCCGGT	45.0	50.0	57.6	56.7	2385 bp	1.0	67.0
ndhF_p5	TGTTTCGATGCAACAGCAAG	TCGAAAGAGGTAAGATCCGGT	45.0	47.6	57.6	58.3	2386 bp	1.0	66.9