Hotspot

rpl32-trnL-UAG

SSC · 111921-112771 · 851 bp

Back to Carduus

Pi 0.00313
MarkerSeek score 56.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
851
variable_sites
5
pi
0.00313
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGAAAGGATATTGGGCGG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.6 58.0 984-986 bp 1.0 77.5
rpl32-trnL-UAG_p2 AAGGAAAGGATATTGGGCGG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.6 58.0 985-987 bp 1.0 77.5
rpl32-trnL-UAG_p3 CGGCGTTAAAAGCTTTGTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 967-969 bp 1.0 77.3
rpl32-trnL-UAG_p4 CGGCGTTAAAAGCTTTGTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 968-970 bp 1.0 77.3
rpl32-trnL-UAG_p5 AAGGAAAGGATATTGGGCGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.6 57.9 994-996 bp 1.0 76.6