Hotspot

ndhF-rpl32

SSC · 110706-111755 · 1050 bp

Back to Carduus

Pi 0.0019

MarkerSeek score 52.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1050
variable_sites: 4
pi: 0.0019
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAAGGAAAGGT	CCGCCCAATATCCTTTCCTT	50.0	50.0	57.0	57.6	1184 bp	1.0	69.7
ndhF-rpl32_p2	CTGGAAGTGGAAGGAAAGGT	TGACAAAGCTTTTAACGCCG	50.0	45.0	57.0	57.6	1201 bp	1.0	69.4
ndhF-rpl32_p3	CTGGAAGTGGAAGGAAAGGT	TTTAACGCCGCCCAATATCC	50.0	50.0	57.0	58.7	1191 bp	1.0	67.1
ndhF-rpl32_p4	CTGGAAGTGGAAGGAAAGGT	GACAAAGCTTTTAACGCCGC	50.0	50.0	57.0	58.9	1200 bp	1.0	65.0
ndhF-rpl32_p5	CTGGAAGTGGAAGGAAAGGT	CTTTTAACGCCGCCCAATATC	50.0	47.6	57.0	58.0	1193 bp	1.0	64.6