Hotspot

ndhC-trnV-UAC

LSC · 49478-50644 · 1167 bp

Back to Carduus

Pi 0.003
MarkerSeek score 62.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1167
variable_sites
7
pi
0.003
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCCTTTGCTAATCGGAGCTA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.1 58.1 1289-1335 bp 1.0 73.8
ndhC-trnV-UAC_p2 CCCTTTGCTAATCGGAGCTAA CCGAGAAAGTCTACGGTTCG 47.6 55.0 57.8 58.1 1289-1335 bp 1.0 71.5
ndhC-trnV-UAC_p3 CTCCGGCCCTTTGCTAATC CCGAGAAAGTCTACGGTTCG 57.9 55.0 58.3 58.1 1295-1341 bp 1.0 70.8
ndhC-trnV-UAC_p4 GGCCCTTTGCTAATCGGAG CCGAGAAAGTCTACGGTTCG 57.9 55.0 58.3 58.1 1291-1337 bp 1.0 70.8
ndhC-trnV-UAC_p5 TTCTCCGGCCCTTTGCTAAT CCGAGAAAGTCTACGGTTCG 50.0 55.0 59.4 58.1 1297-1343 bp 1.0 70.1