Hotspot

petN-psbM

LSC · 9830-10324 · 495 bp

Back to Carduus

Pi 0.00404

MarkerSeek score 46.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 4
pi: 0.00404
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTCTTCATTT	50.0	40.0	58.2	55.0	657-661 bp	1.0	55.1
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTCTTCATTTT	50.0	38.1	58.2	55.8	657-661 bp	1.0	53.7
petN-psbM_p3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTCTTCATTT	50.0	40.0	57.1	55.0	658-662 bp	1.0	49.2
petN-psbM_p4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTCTTCATTT	50.0	40.0	58.9	55.0	649-653 bp	1.0	49.1
petN-psbM_p5	TGGGCTGCTTTAATGGTAGTC	TGCTACTGCACTCTTCATTT	47.6	40.0	58.0	55.0	643-647 bp	1.0	48.3