Hotspot

trnS-GCU-trnC-GCA

LSC · 8521-9141 · 621 bp

Back to Carduus

Pi 0.00322

MarkerSeek score 57.70

Primer available yes

Label trnS-GCU-trnC-GCA

Feature Metadata

label_name: trnS-GCU-trnC-GCA
feature_type: igs
region: LSC
length_bp: 621
variable_sites: 4
pi: 0.00322
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	TCAGAGATTCTCGCGAGTCT	50.0	50.0	58.2	58.0	823-827 bp	1.0	79.0
trnS-GCU-trnC-GCA_p2	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	772-776 bp	1.0	78.6
trnS-GCU-trnC-GCA_p3	ACGGAAAGAGAGGGATTCGA	CACTCAGAGATTCTCGCGAG	50.0	55.0	58.2	57.9	826-830 bp	1.0	78.4
trnS-GCU-trnC-GCA_p4	ACGGAAAGAGAGGGATTCGA	ACTCAGAGATTCTCGCGAGT	50.0	50.0	58.2	58.3	825-829 bp	1.0	77.2
trnS-GCU-trnC-GCA_p5	ACGGAAAGAGAGGGATTCGA	AACACTCAGAGATTCTCGCG	50.0	50.0	58.2	57.7	828-832 bp	1.0	77.1