Hotspot

rps4

LSC · 43892-44497 · 606 bp

Back to Cardiocrinum

Pi 0.00165
MarkerSeek score 59.70
Primer available yes
Label rps4

Feature Metadata

label_name
rps4
feature_type
gene
region
LSC
length_bp
606
variable_sites
1
pi
0.00165
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4_p1 AGAAAAATGTGGATCAAGGCTC AGCCTTGCTTTGTTTATCCT 40.9 40.0 56.9 55.2 786 bp 1.0 40.1
rps4_p2 GAAAAATGTGGATCAAGGCTCT AGCCTTGCTTTGTTTATCCT 40.9 40.0 56.9 55.2 785 bp 1.0 40.1
rps4_p3 AAAATGTGGATCAAGGCTCT AGCCTTGCTTTGTTTATCCT 40.0 40.0 54.9 55.2 783 bp 1.0 40.1
rps4_p4 AAATGTGGATCAAGGCTCTT AGCCTTGCTTTGTTTATCCT 40.0 40.0 54.9 55.2 782 bp 1.0 40.1
rps4_p5 GAAAAATGTGGATCAAGGCTCT AGCCTTGCTTTGTTTATCCTT 40.9 38.1 56.9 56.0 785 bp 1.0 40.1