Hotspot

ndhA

SSC · 117618-119751 · 2134 bp

Back to Cardiocrinum

Pi 0.00141

MarkerSeek score 45.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2134
variable_sites: 3
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACTCAGTCACCATAGGAAACA	ATGGGAGAAGTTGACCGTTG	42.9	50.0	56.3	57.8	2266 bp	1.0	57.0
ndhA_p2	CATGAACTCAGTCACCATAGGA	ATGGGAGAAGTTGACCGTTG	45.5	50.0	57.3	57.8	2271 bp	1.0	56.7
ndhA_p3	TCATGAACTCAGTCACCATAGG	ATGGGAGAAGTTGACCGTTG	45.5	50.0	57.3	57.8	2272 bp	1.0	56.7
ndhA_p4	ACTCAGTCACCATAGGAAACA	GGGAGAAGTTGACCGTTGAA	42.9	50.0	56.3	57.8	2264 bp	1.0	56.4
ndhA_p5	CATGAACTCAGTCACCATAGGA	GGGAGAAGTTGACCGTTGAA	45.5	50.0	57.3	57.8	2269 bp	1.0	56.2