Hotspot

atpB

LSC · 51232-52728 · 1497 bp

Back to Cardiocrinum

Pi 0.002

MarkerSeek score 59.90

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 3
pi: 0.002
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	CAATAGGGGCATGGTTTGGT	TCGGACATATTTTTGTTGATTCGG	50.0	37.5	58.1	58.1	1676 bp	1.0	46.1
atpB_p2	CAATAGGGGCATGGTTTGGT	CGGACATATTTTTGTTGATTCGG	50.0	39.1	58.1	56.8	1675 bp	1.0	45.6
atpB_p3	CAATAGGGGCATGGTTTGGT	CGGACATATTTTTGTTGATTCGGT	50.0	37.5	58.1	58.4	1675 bp	1.0	44.2
atpB_p4	AGCAATAGGGGCATGGTTTG	TCGGACATATTTTTGTTGATTCGG	50.0	37.5	58.5	58.1	1678 bp	1.0	43.0
atpB_p5	AGCAATAGGGGCATGGTTTG	CGGACATATTTTTGTTGATTCGG	50.0	39.1	58.5	56.8	1677 bp	1.0	42.6