Hotspot

ccsA-ndhD

SSC · 113393-113560 · 168 bp

Back to Cardiocrinum

Pi 0.0357
MarkerSeek score 65.20
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
168
variable_sites
6
pi
0.0357
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGTTCATTCACATTAACATCT TCAGTTGACAAGGTACAAGCT 31.8 42.9 53.7 57.2 244 bp 1.0 42.0
ccsA-ndhD_p2 ATGGTTCATTCACATTAACATCT TCAGTTGACAAGGTACAAGCT 30.4 42.9 54.3 57.2 245 bp 1.0 42.0
ccsA-ndhD_p3 TGGTTCATTCACATTAACATCT ATCAGTTGACAAGGTACAAGCT 31.8 40.9 53.7 57.6 245 bp 1.0 42.0
ccsA-ndhD_p4 TGGTTCATTCACATTAACATCT TCAGTTGACAAGGTACAAGC 31.8 45.0 53.7 55.6 244 bp 1.0 42.0
ccsA-ndhD_p5 ACATAGTTATGGTTCATTCACA TCAGTTGACAAGGTACAAGCT 31.8 42.9 53.2 57.2 253 bp 1.0 41.9