Hotspot

trnT-GGU-psbD

LSC · 30602-31676 · 1075 bp

Back to Cardiocrinum

Pi 0.000933
MarkerSeek score 59.60
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1075
variable_sites
1
pi
0.000933
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG CACAAAAACGAAACGGTCCC 55.0 50.0 58.1 58.2 1223-1226 bp 1.0 78.4
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG CCACAAAAACGAAACGGTCC 55.0 50.0 58.1 58.2 1224-1227 bp 1.0 78.4
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG CCCACAAAAACGAAACGGTC 55.0 50.0 58.1 58.2 1225-1228 bp 1.0 78.4
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA CACAAAAACGAAACGGTCCC 50.0 50.0 58.1 58.2 1226-1229 bp 1.0 78.2
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA CCACAAAAACGAAACGGTCC 50.0 50.0 58.1 58.2 1227-1230 bp 1.0 78.2