Hotspot

matK-trnK-UUU

LSC · 3229-4040 · 812 bp

Back to Cardiocrinum

Pi 0.00123

MarkerSeek score 59.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 812
variable_sites: 1
pi: 0.00123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TAGGAAGTGTTGTTGCGGAG	CAATCCGGGTTGCTAACTCA	50.0	50.0	57.8	57.9	912-916 bp	1.0	77.9
matK-trnK-UUU_p2	TAGGAAGTGTTGTTGCGGAG	ACAATCCGGGTTGCTAACTC	50.0	50.0	57.8	57.9	913-917 bp	1.0	77.9
matK-trnK-UUU_p3	TAGGAAGTGTTGTTGCGGAG	ATCCGGGTTGCTAACTCAAC	50.0	50.0	57.8	57.9	910-914 bp	1.0	77.9
matK-trnK-UUU_p4	TAGGAAGTGTTGTTGCGGAG	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.8	58.2	893-897 bp	1.0	77.4
matK-trnK-UUU_p5	TAGGAAGTGTTGTTGCGGAG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.8	58.2	892-896 bp	1.0	77.4