Hotspot

rpl32-trnL-UAG

SSC · 114175-114953 · 779 bp

Back to Cardamine

Pi 0.0462
MarkerSeek score 84.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
779
variable_sites
176
pi
0.0462
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGGACATCGTTGAAAGCTTT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 55.9 57.9 677-908 bp 0.96 72.4
rpl32-trnL-UAG_p2 TTGGACATCGTTGAAAGCTT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 55.9 57.9 678-909 bp 0.96 72.4
rpl32-trnL-UAG_p3 TGGACATCGTTGAAAGCTTT AGCAGCGTGTCTACCAATTT 40.0 45.0 55.9 57.8 670-901 bp 0.96 71.9
rpl32-trnL-UAG_p4 TTGGACATCGTTGAAAGCTT AGCAGCGTGTCTACCAATTT 40.0 45.0 55.9 57.8 671-902 bp 0.96 71.9
rpl32-trnL-UAG_p5 TGGACATCGTTGAAAGCTTT CTAAGAGCAGCGTGTCTACC 40.0 55.0 55.9 57.8 675-906 bp 0.96 71.9