Hotspot

ndhF-rpl32

SSC · 113175-114015 · 841 bp

Back to Cardamine

Pi 0.0357

MarkerSeek score 81.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 841
variable_sites: 176
pi: 0.0357
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGGAGTGGAATGAAAGGG	ACGAATACGCTTTTTCGAGA	55.0	40.0	57.9	55.5	722-966 bp	0.92	68.1
ndhF-rpl32_p2	TGGGAGTGGAATGAAAGGGA	ACGAATACGCTTTTTCGAGA	50.0	40.0	58.2	55.5	721-965 bp	0.92	67.2
ndhF-rpl32_p3	CTGGGAGTGGAATGAAAGGG	ACGAATACGCTTTTTCGAGAT	55.0	38.1	57.9	56.1	722-966 bp	0.92	64.5
ndhF-rpl32_p4	TGGGAGTGGAATGAAAGGGA	ACGAATACGCTTTTTCGAGAT	50.0	38.1	58.2	56.1	721-965 bp	0.92	63.7
ndhF-rpl32_p5	ACTGGGAGTGGAATGAAAGG	ACGAATACGCTTTTTCGAGA	50.0	40.0	57.1	55.5	723-967 bp	0.92	62.1