Hotspot

psaJ-rpl33

LSC · 67277-67710 · 434 bp

Back to Cardamine

Pi 0.0388

MarkerSeek score 79.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 434
variable_sites: 79
pi: 0.0388
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCCAGATGCATTAACATTTCCCT	AACATCTTTACCCTTGGCCA	39.1	45.0	58.1	57.0	449-512 bp	0.96	57.4
psaJ-rpl33_p2	TCCAGATGCATTAACATTTCCC	AACATCTTTACCCTTGGCCA	40.9	45.0	56.7	57.0	449-512 bp	0.96	56.3
psaJ-rpl33_p3	CCAGATGCATTAACATTTCCCT	AACATCTTTACCCTTGGCCA	40.9	45.0	56.7	57.0	448-511 bp	0.96	56.2
psaJ-rpl33_p4	TCCAGATGCATTAACATTTCCCT	GAACATCTTTACCCTTGGCCA	39.1	47.6	58.1	58.2	450-513 bp	0.96	55.8
psaJ-rpl33_p5	TCCAGATGCATTAACATTTCCCT	CGAACATCTTTACCCTTGGC	39.1	50.0	58.1	56.8	451-514 bp	0.96	55.7