Hotspot

psbE-petL

LSC · 64553-65901 · 1349 bp

Back to Cardamine

Pi 0.0279

MarkerSeek score 77.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1349
variable_sites: 193
pi: 0.0279
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	57.9	56.3	596-1531 bp	0.96	76.1
psbE-petL_p2	AATAACCAGCCCGCAATGAA	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	597-1532 bp	0.96	75.5
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	514-1449 bp	0.96	75.5
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	509-1444 bp	0.96	75.1
psbE-petL_p5	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGAA	45.0	42.9	57.9	57.0	596-1531 bp	0.96	74.2