Hotspot

psaA-ycf3

LSC · 41848-42571 · 724 bp

Back to Cardamine

Pi 0.0167

MarkerSeek score 84.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 724
variable_sites: 73
pi: 0.0167
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGACGATCACGAGG	50.0	50.0	57.9	58.0	805-850 bp	1.0	90.4
psaA-ycf3_p2	AGAAATGACCGGGTTTAGCC	ATTGGTTGACGATCACGAGG	50.0	50.0	57.9	58.0	822-867 bp	1.0	90.2
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGCACAGAATTGGTTGACGA	50.0	45.0	57.9	57.7	813-858 bp	1.0	88.5
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	867-912 bp	1.0	88.0
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	869-914 bp	1.0	88.0