Hotspot

trnT-GGU-psbD

LSC · 31502-32738 · 1237 bp

Back to Cardamine

Pi 0.0239

MarkerSeek score 77.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1237
variable_sites: 149
pi: 0.0239
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	TTTACCAAGGGCTATAGTCA	55.0	40.0	58.1	53.0	1092-1331 bp	1.0	52.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.1	53.0	1095-1334 bp	1.0	52.0
trnT-GGU-psbD_p3	CCAAGACGGCCCTTTTAACT	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.1	53.0	1114-1353 bp	1.0	52.0
trnT-GGU-psbD_p4	ACGGCCCTTTTAACTCAGTG	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.1	53.0	1109-1348 bp	1.0	52.0
trnT-GGU-psbD_p5	GACGGCCCTTTTAACTCAGT	TTTACCAAGGGCTATAGTCA	50.0	40.0	57.8	53.0	1110-1349 bp	1.0	52.0