Hotspot

trnS-GCU-trnG-UCC

LSC · 7910-8586 · 677 bp

Back to Cardamine

Pi 0.0314
MarkerSeek score 75.70
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
677
variable_sites
130
pi
0.0314
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAAGCCGCTGTAAGTTTTCG AAGGGTTTTAGTCGACGTCG 50.0 50.0 58.0 57.9 2032-2183 bp 1.0 91.2
trnS-GCU-trnG-UCC_p2 AGCCGCTGTAAGTTTTCGAT AAGGGTTTTAGTCGACGTCG 45.0 50.0 57.9 57.9 2030-2181 bp 1.0 90.8
trnS-GCU-trnG-UCC_p3 AAGCCGCTGTAAGTTTTCGA AAGGGTTTTAGTCGACGTCG 45.0 50.0 58.1 57.9 2031-2182 bp 1.0 90.5
trnS-GCU-trnG-UCC_p4 CAAGCCGCTGTAAGTTTTCG CGTATGGGTGCTTTCACAGA 50.0 50.0 58.0 57.9 1853-2000 bp 0.8 82.9
trnS-GCU-trnG-UCC_p5 AGCCGCTGTAAGTTTTCGAT CGTATGGGTGCTTTCACAGA 45.0 50.0 57.9 57.9 1851-1998 bp 0.8 82.5