Hotspot

trnK-UUU-rps16

LSC · 4321-4993 · 673 bp

Back to Cardamine

Pi 0.0381
MarkerSeek score 81.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
673
variable_sites
141
pi
0.0381
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 150-1096 bp 1.0 91.5
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG TCCCAATGAGCCGTTTATCG 55.0 50.0 58.0 58.1 149-1097 bp 1.0 91.5
trnK-UUU-rps16_p3 TCAGGATCAGTCGTGGTCTT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.1 58.1 146-1092 bp 1.0 91.0
trnK-UUU-rps16_p4 TCAGGATCAGTCGTGGTCTT TCCCAATGAGCCGTTTATCG 50.0 50.0 58.1 58.1 145-1093 bp 1.0 91.0
trnK-UUU-rps16_p5 TTCAGGATCAGTCGTGGTCT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.1 58.1 147-1093 bp 1.0 91.0