Hotspot

trnH-GUG-psbA

LSC · 77-354 · 278 bp

Back to Cardamine

Pi 0.0752

MarkerSeek score 86.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 278
variable_sites: 95
pi: 0.0752
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	235-376 bp	1.0	90.5
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	253-394 bp	1.0	90.3
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	50.0	55.0	58.2	58.1	236-377 bp	1.0	90.3
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	250-391 bp	0.96	88.8
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	251-392 bp	0.96	88.6