Hotspot

ndhH

SSC · 129152-130333 · 1182 bp

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Pi 0.481

MarkerSeek score 80.60

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 479
pi: 0.481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GCCTCGAGACGGAAAAAGAT	GGGGATCCGTCGAATTTCAA	50.0	50.0	58.0	58.0	1821 bp	0.5	59.6
ndhH_p2	GAAGAACTCCTAAAGGGCCG	GGGGATCCGTCGAATTTCAA	55.0	50.0	58.0	58.0	1762 bp	0.5	59.5
ndhH_p3	GCCTCGAGACGGAAAAAGAT	TACTAGGAAAACGCCAACGG	50.0	50.0	58.0	57.9	1710 bp	0.5	59.2
ndhH_p4	GCCTCGAGACGGAAAAAGAT	AGCTGGATATTCGGGAGTCA	50.0	50.0	58.0	57.9	1635 bp	0.5	59.2
ndhH_p5	GAAGAACTCCTAAAGGGCCG	TACTAGGAAAACGCCAACGG	55.0	50.0	58.0	57.9	1651 bp	0.5	59.1