Hotspot

ndhG

SSC · 125435-126013 · 579 bp

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Pi 0.487

MarkerSeek score 79.10

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 579
variable_sites: 227
pi: 0.487
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ATAGCCAACCCAATAGCTGC	TAAGTTTTTCCCGGTCGGAC	50.0	50.0	58.0	57.8	1045 bp	0.5	58.5
ndhG_p2	CACCCGGTTGGATTGACTAG	TAAGTTTTTCCCGGTCGGAC	55.0	50.0	58.0	57.8	752 bp	0.5	58.5
ndhG_p3	ACCCGGTTGGATTGACTAGA	TAAGTTTTTCCCGGTCGGAC	50.0	50.0	58.1	57.8	751 bp	0.5	58.2
ndhG_p4	CCAACCCAATAGCTGCTTCA	TAAGTTTTTCCCGGTCGGAC	50.0	50.0	58.2	57.8	1041 bp	0.5	57.4
ndhG_p5	ATAGCCAACCCAATAGCTGC	AGAGAGAACTGGAATGGGGT	50.0	50.0	58.0	57.6	1331 bp	0.5	57.1