Hotspot

ndhD

SSC · 122808-124292 · 1485 bp

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Pi 0.468

MarkerSeek score 82.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1485
variable_sites: 632
pi: 0.468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CGAGGTCCAATACATGACGG	GCAGCTATTGGGTTGGCTAT	55.0	50.0	58.1	58.0	2889 bp	0.5	58.7
ndhD_p2	CGAGGTCCAATACATGACGG	GGCATGAAACAACTCGAAGC	55.0	50.0	58.1	58.0	2346 bp	0.5	58.6
ndhD_p3	GGGAGCAGTATGGGCTAATG	GCAGCTATTGGGTTGGCTAT	55.0	50.0	57.8	58.0	2730 bp	0.5	58.4
ndhD_p4	GGGAGCAGTATGGGCTAATG	GGCATGAAACAACTCGAAGC	55.0	50.0	57.8	58.0	2187 bp	0.5	58.2
ndhD_p5	CGAGGTCCAATACATGACGG	CGGGTTTTTCTAGCCCAAGT	55.0	50.0	58.1	58.1	2223 bp	0.5	58.1