Hotspot

ccsA

SSC · 121507-122538 · 1032 bp

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Pi 0.474

MarkerSeek score 79.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 1032
variable_sites: 380
pi: 0.474
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	TTACGCCAGATGTTCCATGG	50.0	50.0	58.0	58.0	1620 bp	0.5	59.6
ccsA_p2	TGAAATCGGTAGACACGCTG	TTACGCCAGATGTTCCATGG	50.0	50.0	58.0	58.0	1619 bp	0.5	59.6
ccsA_p3	GTGAAATCGGTAGACACGCT	TTGGCAGGAACGAGTTTTGA	50.0	45.0	58.0	58.0	1887 bp	0.5	59.6
ccsA_p4	TGAAATCGGTAGACACGCTG	TTGGCAGGAACGAGTTTTGA	50.0	45.0	58.0	58.0	1886 bp	0.5	59.6
ccsA_p5	GTGAAATCGGTAGACACGCT	TATCTTGACGAAATGGGCGG	50.0	50.0	58.0	58.1	1851 bp	0.5	59.5