Hotspot

ndhF-rpl32

SSC · 118957-119732 · 776 bp

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Pi 0.421

MarkerSeek score 80.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 776
variable_sites: 322
pi: 0.421
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGCAATAGGTCGTGTGAAC	CGACAAAGCTTTCAAGGCTG	50.0	50.0	57.9	58.0	1476 bp	0.5	58.9
ndhF-rpl32_p2	AGGCAATAGGTCGTGTGAAC	TTCAAGGCTGCCCAATATCC	50.0	50.0	57.9	57.9	1466 bp	0.5	58.7
ndhF-rpl32_p3	AGGCAATAGGTCGTGTGAAC	TTGTGACAAGTAGGCCGATG	50.0	50.0	57.9	57.9	2003 bp	0.5	58.5
ndhF-rpl32_p4	TGACAAGCATTCGAGGCAAT	CGACAAAGCTTTCAAGGCTG	45.0	50.0	58.2	58.0	1489 bp	0.5	58.3
ndhF-rpl32_p5	GGGTCGATCAAGTGTCCAAA	CGACAAAGCTTTCAAGGCTG	50.0	50.0	57.8	58.0	1234 bp	0.5	58.2