Hotspot

rpl22-rps19

LSC · 89646-89732 · 87 bp

Back to Carallia

Pi 0.0575

MarkerSeek score 82.20

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 87
variable_sites: 5
pi: 0.0575
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTGCTTTGTAGGCGGATAT	GTGGGCCATAAATTGGGAGA	50.0	50.0	58.1	57.5	251-290 bp	1.0	76.8
rpl22-rps19_p2	GCCCGCGAATTTGATCAATT	GTGGGCCATAAATTGGGAGA	45.0	50.0	57.8	57.5	277-316 bp	1.0	76.1
rpl22-rps19_p3	CGTGCTTTGTAGGCGGATAT	GGTGGGCCATAAATTGGGAG	50.0	55.0	58.1	58.6	252-291 bp	1.0	75.7
rpl22-rps19_p4	GCCCGCGAATTTGATCAATT	GGTGGGCCATAAATTGGGAG	45.0	55.0	57.8	58.6	278-317 bp	1.0	75.0
rpl22-rps19_p5	CGTGCTTTGTAGGCGGATAT	TAACAGATCGTATGGTGGGC	50.0	50.0	58.1	57.1	265-304 bp	1.0	73.1