primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	rpl22-rps19	1	CGTGCTTTGTAGGCGGATAT	GTGGGCCATAAATTGGGAGA	20	20	50.0	50.0	58.1	57.5	0.0	10.7	0.586736	89646	89732	251-290	1.000000	76.8
rpl22-rps19_p2	rpl22-rps19	2	GCCCGCGAATTTGATCAATT	GTGGGCCATAAATTGGGAGA	20	20	45.0	50.0	57.8	57.5	8.2	10.7	0.660926	89646	89732	277-316	1.000000	76.1
rpl22-rps19_p3	rpl22-rps19	3	CGTGCTTTGTAGGCGGATAT	GGTGGGCCATAAATTGGGAG	20	20	50.0	55.0	58.1	58.6	0.0	10.7	0.721017	89646	89732	252-291	1.000000	75.7
rpl22-rps19_p4	rpl22-rps19	4	GCCCGCGAATTTGATCAATT	GGTGGGCCATAAATTGGGAG	20	20	45.0	55.0	57.8	58.6	8.2	10.7	0.795208	89646	89732	278-317	1.000000	75.0
rpl22-rps19_p5	rpl22-rps19	5	CGTGCTTTGTAGGCGGATAT	TAACAGATCGTATGGTGGGC	20	20	50.0	50.0	58.1	57.1	0.0	0.0	1.036840	89646	89732	265-304	1.000000	73.1
ndhF_p1	ndhF	1	ACGAAGGGGACTAAACAGGA	AACCACTTCCGACTTCTTGA	20	20	50.0	45.0	58.0	56.7	0.0	0.0	1.312995	116692	118956	2885-2885	0.500000	49.5
ndhF_p2	ndhF	2	ACGGAAGAAAGGGAGGAAGA	AACCACTTCCGACTTCTTGA	20	20	50.0	45.0	58.0	56.7	0.0	0.0	1.318823	116692	118956	2936-2936	0.500000	49.4
ndhF_p3	ndhF	3	GCAGAATACCGTCACCCATT	AACCACTTCCGACTTCTTGA	20	20	50.0	45.0	58.0	56.7	0.0	0.0	1.331574	116692	118956	2986-2986	0.500000	49.3
ndhF_p4	ndhF	4	GATCCGGACAAAATCGACGA	AACCACTTCCGACTTCTTGA	20	20	50.0	45.0	58.1	56.7	6.1	0.0	1.375915	116692	118956	2807-2807	0.500000	49.0
ndhF_p5	ndhF	5	AGGGGACTAAACAGGAACCA	AACCACTTCCGACTTCTTGA	20	20	50.0	45.0	57.9	56.7	0.0	0.0	1.420839	116692	118956	2881-2881	0.500000	48.6
ndhF-rpl32_p1	ndhF-rpl32	1	AGGCAATAGGTCGTGTGAAC	CGACAAAGCTTTCAAGGCTG	20	20	50.0	50.0	57.9	58.0	0.0	9.2	0.136866	118957	119732	1476-1476	0.500000	58.9
ndhF-rpl32_p2	ndhF-rpl32	2	AGGCAATAGGTCGTGTGAAC	TTCAAGGCTGCCCAATATCC	20	20	50.0	50.0	57.9	57.9	0.0	16.6	0.166540	118957	119732	1466-1466	0.500000	58.7
ndhF-rpl32_p3	ndhF-rpl32	3	AGGCAATAGGTCGTGTGAAC	TTGTGACAAGTAGGCCGATG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.188068	118957	119732	2003-2003	0.500000	58.5
ndhF-rpl32_p4	ndhF-rpl32	4	TGACAAGCATTCGAGGCAAT	CGACAAAGCTTTCAAGGCTG	20	20	45.0	50.0	58.2	58.0	12.8	9.2	0.217014	118957	119732	1489-1489	0.500000	58.3
ndhF-rpl32_p5	ndhF-rpl32	5	GGGTCGATCAAGTGTCCAAA	CGACAAAGCTTTCAAGGCTG	20	20	50.0	50.0	57.8	58.0	0.0	9.2	0.221016	118957	119732	1234-1234	0.500000	58.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CAGCCTTGAAAGCTTTGTCG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	18.3	0.0	0.049934	119994	121379	1510-1510	0.500000	59.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CAGCCTTGAAAGCTTTGTCG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	18.3	0.0	0.051305	119994	121379	1511-1511	0.500000	59.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CAGCCTTGAAAGCTTTGTCG	AAAAGCCCCGAAGTAGCAAA	20	20	50.0	45.0	58.0	58.0	18.3	0.0	0.052398	119994	121379	2027-2027	0.500000	59.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CAGCCTTGAAAGCTTTGTCG	AAAGCCCCGAAGTAGCAAAA	20	20	50.0	45.0	58.0	58.0	18.3	0.0	0.052398	119994	121379	2026-2026	0.500000	59.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGATATTGGGCAGCCTTGAA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.9	58.0	12.4	0.0	0.079608	119994	121379	1520-1520	0.500000	59.4
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	TTACGCCAGATGTTCCATGG	20	20	50.0	50.0	58.0	58.0	0.0	5.0	0.044788	121507	122538	1620-1620	0.500000	59.6
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	TTACGCCAGATGTTCCATGG	20	20	50.0	50.0	58.0	58.0	0.0	5.0	0.046158	121507	122538	1619-1619	0.500000	59.6
ccsA_p3	ccsA	3	GTGAAATCGGTAGACACGCT	TTGGCAGGAACGAGTTTTGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.053392	121507	122538	1887-1887	0.500000	59.6
ccsA_p4	ccsA	4	TGAAATCGGTAGACACGCTG	TTGGCAGGAACGAGTTTTGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.054762	121507	122538	1886-1886	0.500000	59.6
ccsA_p5	ccsA	5	GTGAAATCGGTAGACACGCT	TATCTTGACGAAATGGGCGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.059726	121507	122538	1851-1851	0.500000	59.5
ccsA-ndhD_p1	ccsA-ndhD	1	TTTGCTACTTCGGGGCTTTT	TTACGCCAGATGTTCCATGG	20	20	45.0	50.0	58.0	58.0	0.0	5.0	0.047251	122539	122807	1103-1103	0.500000	59.6
ccsA-ndhD_p2	ccsA-ndhD	2	TTTTGCTACTTCGGGGCTTT	TTACGCCAGATGTTCCATGG	20	20	45.0	50.0	58.0	58.0	0.0	5.0	0.047251	122539	122807	1104-1104	0.500000	59.6
ccsA-ndhD_p3	ccsA-ndhD	3	TTTGCTACTTCGGGGCTTTT	TTGGCAGGAACGAGTTTTGA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.055855	122539	122807	1370-1370	0.500000	59.6
ccsA-ndhD_p4	ccsA-ndhD	4	TTTTGCTACTTCGGGGCTTT	TTGGCAGGAACGAGTTTTGA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.055855	122539	122807	1371-1371	0.500000	59.6
ccsA-ndhD_p5	ccsA-ndhD	5	TTTGCTACTTCGGGGCTTTT	TATCTTGACGAAATGGGCGG	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.062189	122539	122807	1334-1334	0.500000	59.5
ndhD_p1	ndhD	1	CGAGGTCCAATACATGACGG	GCAGCTATTGGGTTGGCTAT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.160176	122808	124292	2889-2889	0.500000	58.7
ndhD_p2	ndhD	2	CGAGGTCCAATACATGACGG	GGCATGAAACAACTCGAAGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.177955	122808	124292	2346-2346	0.500000	58.6
ndhD_p3	ndhD	3	GGGAGCAGTATGGGCTAATG	GCAGCTATTGGGTTGGCTAT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.204245	122808	124292	2730-2730	0.500000	58.4
ndhD_p4	ndhD	4	GGGAGCAGTATGGGCTAATG	GGCATGAAACAACTCGAAGC	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.222024	122808	124292	2187-2187	0.500000	58.2
ndhD_p5	ndhD	5	CGAGGTCCAATACATGACGG	CGGGTTTTTCTAGCCCAAGT	20	20	55.0	50.0	58.1	58.1	0.0	3.6	0.241421	122808	124292	2223-2223	0.500000	58.1
ndhD-psaC_p1	ndhD-psaC	1	AGAACCTGCGGATATCGGTA	GCAGCTATTGGGTTGGCTAT	20	20	50.0	50.0	58.0	58.0	11.5	0.0	0.021997	124293	124437	759-759	0.500000	59.8
ndhD-psaC_p2	ndhD-psaC	2	AGAACCTGCGGATATCGGTA	GGCATGAAACAACTCGAAGC	20	20	50.0	50.0	58.0	58.0	11.5	0.0	0.039776	124293	124437	216-216	0.500000	59.7
ndhD-psaC_p3	ndhD-psaC	3	GCGTATCTGGCAACCATGTA	GCAGCTATTGGGTTGGCTAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.065013	124293	124437	1414-1414	0.500000	59.5
ndhD-psaC_p4	ndhD-psaC	4	GCGTATCTGGCAACCATGTA	GGCATGAAACAACTCGAAGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.082792	124293	124437	871-871	0.500000	59.3
ndhD-psaC_p5	ndhD-psaC	5	TTACGAGTAACTGGCCAAGC	GCAGCTATTGGGTTGGCTAT	20	20	50.0	50.0	57.9	58.0	7.0	0.0	0.102067	124293	124437	1031-1031	0.500000	59.2
ndhE-ndhG_p1	ndhE-ndhG	1	ATAGCCAACCCAATAGCTGC	GCTTTGATAGGTGCGATTGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.124158	125235	125434	472-472	0.500000	59.0
ndhE-ndhG_p2	ndhE-ndhG	2	ATAGCCAACCCAATAGCTGC	GTTGGGGATGGGGTTACTTC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.152441	125235	125434	691-691	0.500000	58.8
ndhE-ndhG_p3	ndhE-ndhG	3	ATAGCCAACCCAATAGCTGC	TAAGTTTTTCCCGGTCGGAC	20	20	50.0	50.0	58.0	57.8	0.0	11.4	0.181443	125235	125434	1045-1045	0.500000	58.5
ndhE-ndhG_p4	ndhE-ndhG	4	ATAGCCAACCCAATAGCTGC	TTGCTGTGGCTCGTCAATAA	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.190511	125235	125434	456-456	0.500000	58.5
ndhE-ndhG_p5	ndhE-ndhG	5	GTTGGACAGGCAGATTCACA	GCTTTGATAGGTGCGATTGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.214821	125235	125434	966-966	0.500000	58.3
ndhG_p1	ndhG	1	ATAGCCAACCCAATAGCTGC	TAAGTTTTTCCCGGTCGGAC	20	20	50.0	50.0	58.0	57.8	0.0	11.4	0.181443	125435	126013	1045-1045	0.500000	58.5
ndhG_p2	ndhG	2	CACCCGGTTGGATTGACTAG	TAAGTTTTTCCCGGTCGGAC	20	20	55.0	50.0	58.0	57.8	0.0	11.4	0.192501	125435	126013	752-752	0.500000	58.5
ndhG_p3	ndhG	3	ACCCGGTTGGATTGACTAGA	TAAGTTTTTCCCGGTCGGAC	20	20	50.0	50.0	58.1	57.8	0.0	11.4	0.221341	125435	126013	751-751	0.500000	58.2
ndhG_p4	ndhG	4	CCAACCCAATAGCTGCTTCA	TAAGTTTTTCCCGGTCGGAC	20	20	50.0	50.0	58.2	57.8	7.8	11.4	0.330214	125435	126013	1041-1041	0.500000	57.4
ndhG_p5	ndhG	5	ATAGCCAACCCAATAGCTGC	AGAGAGAACTGGAATGGGGT	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.363980	125435	126013	1331-1331	0.500000	57.1
ndhG-ndhI_p1	ndhG-ndhI	1	CACCCGGTTGGATTGACTAG	TGCTCCCTATGGTAACTGGT	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.288071	126014	126415	1621-1621	0.500000	57.7
ndhG-ndhI_p2	ndhG-ndhI	2	ACCCGGTTGGATTGACTAGA	TGCTCCCTATGGTAACTGGT	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.316911	126014	126415	1620-1620	0.500000	57.5
ndhG-ndhI_p3	ndhG-ndhI	3	CACCCGGTTGGATTGACTAG	GATCACCTTATCCCACGCAA	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.349759	126014	126415	1542-1542	0.500000	57.2
ndhG-ndhI_p4	ndhG-ndhI	4	CACCCGGTTGGATTGACTAG	ACCTTATCCCACGCAAATCG	20	20	55.0	50.0	58.0	58.3	0.0	0.0	0.360481	126014	126415	1538-1538	0.500000	57.1
ndhG-ndhI_p5	ndhG-ndhI	5	GCAATCGCACCTATCAAAGC	TGCTCCCTATGGTAACTGGT	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.374936	126014	126415	1462-1462	0.500000	57.0
ndhA_p1	ndhA	1	ACCAATGTACCTTGCAGCTC	ACCTCATGGCTGGATCGATA	20	20	50.0	50.0	58.2	58.0	5.4	0.0	0.189071	127002	129150	2989-2989	0.500000	58.5
ndhA_p2	ndhA	2	ACCAATGTACCTTGCAGCTC	TACCTCATGGCTGGATCGAT	20	20	50.0	50.0	58.2	58.0	5.4	0.0	0.189071	127002	129150	2990-2990	0.500000	58.5
ndhA_p3	ndhA	3	GACCAATGTACCTTGCAGCT	ACCTCATGGCTGGATCGATA	20	20	50.0	50.0	58.2	58.0	5.4	0.0	0.189071	127002	129150	2990-2990	0.500000	58.5
ndhA_p4	ndhA	4	GACCAATGTACCTTGCAGCT	TACCTCATGGCTGGATCGAT	20	20	50.0	50.0	58.2	58.0	5.4	0.0	0.189071	127002	129150	2991-2991	0.500000	58.5
ndhA_p5	ndhA	5	ACCAGTTACCATAGGGAGCA	ACCTCATGGCTGGATCGATA	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.279351	127002	129150	2941-2941	0.500000	57.8
ndhH_p1	ndhH	1	GCCTCGAGACGGAAAAAGAT	GGGGATCCGTCGAATTTCAA	20	20	50.0	50.0	58.0	58.0	15.7	5.5	0.044221	129152	130333	1821-1821	0.500000	59.6
ndhH_p2	ndhH	2	GAAGAACTCCTAAAGGGCCG	GGGGATCCGTCGAATTTCAA	20	20	55.0	50.0	58.0	58.0	0.0	5.5	0.058269	129152	130333	1762-1762	0.500000	59.5
ndhH_p3	ndhH	3	GCCTCGAGACGGAAAAAGAT	TACTAGGAAAACGCCAACGG	20	20	50.0	50.0	58.0	57.9	15.7	0.0	0.094707	129152	130333	1710-1710	0.500000	59.2
ndhH_p4	ndhH	4	GCCTCGAGACGGAAAAAGAT	AGCTGGATATTCGGGAGTCA	20	20	50.0	50.0	58.0	57.9	15.7	0.0	0.099644	129152	130333	1635-1635	0.500000	59.2
ndhH_p5	ndhH	5	GAAGAACTCCTAAAGGGCCG	TACTAGGAAAACGCCAACGG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.108755	129152	130333	1651-1651	0.500000	59.1
ndhH-rps15_p1	ndhH-rps15	1	ATCGATCCAGCCATGAGGTA	GGGGATCCGTCGAATTTCAA	20	20	50.0	50.0	58.0	58.0	0.0	5.5	0.048137	130334	130444	848-848	0.500000	59.6
ndhH-rps15_p2	ndhH-rps15	2	TATCGATCCAGCCATGAGGT	GGGGATCCGTCGAATTTCAA	20	20	50.0	50.0	58.0	58.0	0.0	5.5	0.048137	130334	130444	849-849	0.500000	59.6
ndhH-rps15_p3	ndhH-rps15	3	ATCGATCCAGCCATGAGGTA	TACTAGGAAAACGCCAACGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.098623	130334	130444	737-737	0.500000	59.2
ndhH-rps15_p4	ndhH-rps15	4	TATCGATCCAGCCATGAGGT	TACTAGGAAAACGCCAACGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.098623	130334	130444	738-738	0.500000	59.2
ndhH-rps15_p5	ndhH-rps15	5	ATCGATCCAGCCATGAGGTA	AGCTGGATATTCGGGAGTCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.103561	130334	130444	662-662	0.500000	59.2
rps15-ycf1_p1	rps15-ycf1	1	TTGAAATTCGACGGATCCCC	GCGAGTCGGTTCAGTATGTT	20	20	50.0	50.0	58.0	58.0	6.1	15.5	0.040511	130709	131158	573-573	0.500000	59.7
rps15-ycf1_p2	rps15-ycf1	2	GATGAGTCGAAGAACGCCAT	GCGAGTCGGTTCAGTATGTT	20	20	50.0	50.0	58.1	58.0	0.0	15.5	0.079935	130709	131158	969-969	0.500000	59.4
rps15-ycf1_p3	rps15-ycf1	3	CCGTTGGCGTTTTCCTAGTA	GCGAGTCGGTTCAGTATGTT	20	20	50.0	50.0	57.9	58.0	0.0	15.5	0.090997	130709	131158	684-684	0.500000	59.3
rps15-ycf1_p4	rps15-ycf1	4	TGACTCCCGAATATCCAGCT	GCGAGTCGGTTCAGTATGTT	20	20	50.0	50.0	57.9	58.0	0.0	15.5	0.095935	130709	131158	759-759	0.500000	59.2
rps15-ycf1_p5	rps15-ycf1	5	TCCGCCATAAAAGGACCAAG	GCGAGTCGGTTCAGTATGTT	20	20	50.0	50.0	57.9	58.0	0.0	15.5	0.130614	130709	131158	1253-1253	0.500000	59.0